ミウラ　ミチ   Michi Miura   三浦　未知    所属   川崎医科大学  医学部 基礎医学　微生物学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The human leukemia virus HTLV-1 alters the structure and transcription of host chromatin in cis.
掲載誌名	正式名：eLife 略　 称：Elife ISSNコード：2050084X/2050084X
掲載区分	国外
巻・号・頁	7,pp.e36245
著者・共著者	Melamed Anat, Yaguchi Hiroko, Miura Michi, Witkover Aviva, Fitzgerald Tomas W, Birney Ewan, Bangham Charles RM
発行年月	2018/06
概要	Chromatin looping controls gene expression by regulating promoter-enhancer contacts, the spread of epigenetic modifications, and the segregation of the genome into transcriptionally active and inactive compartments. We studied the impact on the structure and expression of host chromatin by the human retrovirus HTLV-1. We show that HTLV-1 disrupts host chromatin structure by forming loops between the provirus and the host genome; certain loops depend on the critical chromatin architectural protein CTCF, which we recently discovered binds to the HTLV-1 provirus. We show that the provirus causes two distinct patterns of abnormal transcription of the host genome in cis: bidirectional transcription in the host genome immediately flanking the provirus, and clone-specific transcription in cis at non-contiguous loci up to >300 kb from the integration site. We conclude that HTLV-1 causes insertional mutagenesis up to the megabase range in the host genome in >104 persistently-maintained HTLV-1+ T-cell clones in vivo.
DOI	10.7554/eLife.36245
PMID	29941091