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ミウラ ミチ
Michi Miura
三浦 未知 所属 川崎医科大学 医学部 基礎医学 微生物学 職種 助教 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | A single protofilament is sufficient to support unidirectional walking of dynein and kinesin. |
| 掲載誌名 | 正式名：PLOS One 略 称：PLoS One ISSNコード：19326203/19326203 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 7(8),pp.e42990 |
| 著者・共著者 | Shibata Keitaro, Miura Michi, Watanabe Yuta, Saito Kei, Nishimura Atsuko, Furuta Ken'ya, Toyoshima Yoko Y |
| 担当区分 | 2nd著者 |
| 発行年月 | 2012/08 |
| 概要 | Cytoplasmic dynein and kinesin are two-headed microtubule motor proteins that move in opposite directions on microtubules. It is known that kinesin steps by a 'hand-over-hand' mechanism, but it is unclear by which mechanism dynein steps. Because dynein has a completely different structure from that of kinesin and its head is massive, it is suspected that dynein uses multiple protofilaments of microtubules for walking. One way to test this is to ask whether dynein can step along a single protofilament. Here, we examined dynein and kinesin motility on zinc-induced tubulin sheets (zinc-sheets) which have only one protofilament available as a track for motor proteins. Single molecules of both dynein and kinesin moved at similar velocities on zinc-sheets compared to microtubules, clearly demonstrating that dynein and kinesin can walk on a single protofilament and multiple rows of parallel protofilaments are not essential for their motility. Considering the size and the motile properties of dynein, we suggest that dynein may step by an inchworm mechanism rather than a hand-over-hand mechanism. |
| DOI | 10.1371/journal.pone.0042990 |
| PMID | 22900078 |