ミウラ　ミチ   Michi Miura   三浦　未知    所属   川崎医科大学  医学部 基礎医学　微生物学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Nucleotide-dependent behavior of single molecules of cytoplasmic dynein on microtubules in vitro.
掲載誌名	正式名：FEBS letters 略　 称：FEBS Lett ISSNコード：18733468/00145793
掲載区分	国外
巻・号・頁	584(11),pp.2351-5
著者・共著者	Miura Michi, Matsubara Aiko, Kobayashi Takuya, Edamatsu Masaki, Toyoshima Yoko Y
担当区分	筆頭著者
発行年月	2010/06
概要	We visualized the nucleotide-dependent behavior of single molecules of mammalian native cytoplasmic dynein using fragments of dynactin p150 with or without its N-terminal microtubule binding domain. The results indicate that the binding affinity of dynein for microtubules is high in AMP-PNP, middle in ADP or no nucleotide, and low in ADP.Pi conditions. It is also demonstrated that the microtubule binding domain of dynactin p150 maintains the association of dynein with microtubules without altering the motile property of dynein in the weak binding state. In addition, we observed bidirectional movement of dynein in the presence of ATP as well as in ADP/Vi condition, suggesting that the bidirectional movement is driven by diffusion rather than active transport.
DOI	10.1016/j.febslet.2010.04.016
PMID	20394748