ヤノ　シュウヤ   Shuya Yano   矢野　修也    所属   川崎医科大学  医学部 臨床医学　消化器外科学    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Immuno-hyperthermia effected by antibody-conjugated nanoparticles selectively targets and eradicates individual cancer cells.
掲載誌名	正式名：Cell cycle (Georgetown, Tex.) 略　 称：Cell Cycle ISSNコード：15514005/15514005
掲載区分	国外
巻・号・頁	20(13),pp.1221-1230
著者・共著者	Kagawa Tetsuya, Matsumi Yuki, Aono Hiromichi, Ohara Toshiaki, Tazawa Hiroshi, Shigeyasu Kunitoshi, Yano Shuya, Takeda Sho, Komatsu Yasuhiro, Hoffman Robert M, Fujiwara Toshiyoshi, Kishimoto Hiroyuki
発行年月	2021/07
概要	Hyperthermia has been used for cancer therapy for a long period of time, but has shown limited clinical efficacy. Induction-heating hyperthermia using the combination of magnetic nanoparticles (MNPs) and an alternating magnetic field (AMF), termed magnetic hyperthermia (MHT), has previously shown efficacy in an orthotopic mouse model of disseminated gastric cancer. In the present study, superparamagnetic iron oxide nanoparticles (SPIONs), a type of MNP, were conjugated with an anti-HER2 antibody, trastuzumab and termed anti-HER2-antibody-linked SPION nanoparticles (anti-HER2 SPIONs). Anti-HER2 SPIONs selectively targeted HER2-expressing cancer cells co-cultured along with normal fibroblasts and HER2-negative cancer cells and caused apoptosis only in the HER2-expressing individual cancer cells. The results of the present study show proof-of-concept of a novel hyperthermia technology, immuno-MHT for selective cancer therapy, that targets individual cancer cells.Abbreviations: AMF: alternating magnetic field; DDW: double distilled water; DMEM: Dulbecco's Modified Eagle's; Medium; f: frequency; FBS: fetal bovine serum; FITC: Fluorescein isothiocyanate; GFP: green fluorescent protein; H: amplitude; Hsp: heat shock protein; MHT: magnetic hyperthermia; MNPs: magnetic nanoparticles; PI: propidium iodide; RFP: red fluorescent protein; SPION: superparamagnetic iron oxide (Fe3O4) nanoparticle.
DOI	10.1080/15384101.2021.1915604
PMID	34148497