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Yuma Sakamoto
Department Kawasaki Medical School Kawasaki Medical School, Department of Immunology and Molecular Genetics, Position Instructor |
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| Article types | 原著 |
| Language | English |
| Peer review | Peer reviewed |
| Title | 7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff's reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section. |
| Journal | Formal name：Biotechnic & histochemistry : official publication of the Biological Stain Commission Abbreviation：Biotech Histochem ISSN code：14737760/10520295 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 98(1),pp.54-61 |
| Author and coauthor | Hiroshi Takase, Takayuki Murase, Daisuke Hachisuka, Yuma Sakamoto, Mariko Sugiura, Satsuki Nakano, Keiichiro Fujii, Ayako Masaki, Hiroshi Inagaki |
| Publication date | 2023/01 |
| Summary | An aqueous 7-amino-4-methylcoumarin (AMC) solution exhibits strong fluorescence under ultraviolet (UV) light and can be used as a Schiff reagent to visualize aldehydes. We investigated hemalum and eosin (H & E) and AMC staining for histological and pathological analysis. Sections of normal and lesioned human tissues were stained with combined H & E/AMC staining. After H & E/AMC staining, the H & E morphology was preserved under bright field microscopy. The AMC fluorescent signals observed under UV light were intense and the staining pattern was identical to that obtained by periodic acid-Schiff (PAS) staining. AMC staining of archived H & E sections also was successful. Diastase digestion differentiated glycogen from other AMC positive elements. Using H & E/AMC staining, mucus-rich adenocarcinoma cells, amebic trophozoites and fungal hyphae were visualized clearly under UV excitation. Using H & E/AMC staining, H & E and PAS-like histological imaging can be obtained using a single tissue section. H & E/AMC is useful for pathologic diagnosis especially when information from PAS staining is critical, the number of tissue sections is limited and/or the lesion in question is small. |
| DOI | 10.1080/10520295.2022.2101144 |
| PMID | 35904399 |