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Yuma Sakamoto
Department Kawasaki Medical School Kawasaki Medical School, Department of Immunology and Molecular Genetics, Position Instructor |
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| Article types | 原著 |
| Language | English |
| Peer review | Peer reviewed |
| Title | Improved clonality detection in B-cell lymphoma using a semi-nested modification of the BIOMED-2 PCR assay for IGH rearrangement: A paraffin-embedded tissue study. |
| Journal | Formal name：Pathology international Abbreviation：Pathol Int ISSN code：14401827/13205463 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 67(9),pp.453-460 |
| Author and coauthor | Yuma Sakamoto, Ayako Masaki, Satsuki Aoyama, Shusen Han, Kosuke Saida, Kana Fujii, Hisashi Takino, Takayuki Murase, Shinsuke Iida, Hiroshi Inagaki |
| Publication date | 2017/09 |
| Summary | The BIOMED-2 PCR protocol for targeting the IGH gene is widely employed for detecting clonality in B-cell malignancies. Unfortunately, the detection of clonality with this method is not very sensitive when paraffin sections are used as a DNA source. To increase the sensitivity, we devised a semi-nested modification of a JH consensus primer. The clonality detection rates of three assays were compared: the standard BIOMED-2, BIOMED-2 assay followed by BIOMED-2 re-amplification, and BIOMED-2 assay followed by semi-nested BIOMED-2. We tested more than 100 cases using paraffin-embedded tissues of various B-cell lymphomas, and found that the clonality detection rates with the above three assays were 63.9%, 79.6%, and 88.0%, respectively. While BIOMED-2 re-amplification was significantly more sensitive than the standard BIOMED-2, the semi-nested BIOMED-2 was significantly more sensitive than both the standard BIOMED-2 and BIOMED-2 re-amplification. An increase in sensitivity was observed in all lymphoma subtypes examined. In conclusion, tumor clonality may be detected in nearly 90% of B-cell lymphoma cases with semi-nested BIOMED-2. This ancillary assay may be useful when the standard BIOMED-2 fails to detect clonality in histopathologically suspected B-cell lymphomas. |
| DOI | 10.1111/pin.12566 |
| PMID | 28868745 |