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ヤマサキ アキラ
Akira Yamasaki
山崎 晃 所属 川崎医科大学 医学部 応用医学 衛生学 職種 助教 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans. |
| 掲載誌名 | 正式名：International journal of molecular sciences 略 称：Int J Mol Sci ISSNコード：14220067/14220067 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 22(18),pp.9810-9810 |
| 著者・共著者 | Yamasaki Akira, Suzuki Michiyo, Funayama Tomoo, Moriwaki Takahito, Sakashita Tetsuya, Kobayashi Yasuhiko, Zhang-Akiyama Qiu-Mei |
| 担当区分 | 筆頭著者 |
| 発行年月 | 2021/09 |
| 概要 | Radiation damages many cellular components and disrupts cellular functions, and was previously reported to impair locomotion in the model organism Caenorhabditis elegans. However, the response to even higher doses is not clear. First, to investigate the effects of high-dose radiation on the locomotion of C. elegans, we investigated the dose range that reduces whole-body locomotion or leads to death. Irradiation was performed in the range of 0-6 kGy. In the crawling analysis, motility decreased after irradiation in a dose-dependent manner. Exposure to 6 kGy of radiation affected crawling on agar immediately and caused the complete loss of motility. Both γ-rays and carbon-ion beams significantly reduced crawling motility at 3 kGy. Next, swimming in buffer was measured as a motility index to assess the response over time after irradiation and motility similarly decreased. However, swimming partially recovered 6 h after irradiation with 3 kGy of γ-rays. To examine the possibility of a recovery mechanism, in situ GFP reporter assay of the autophagy-related gene lgg-1 was performed. The fluorescence intensity was stronger in the anterior half of the body 7 h after irradiation with 3 kGy of γ-rays. GFP::LGG-1 induction was observed in the pharynx, neurons along the body, and the intestine. Furthermore, worms were exposed to region-specific radiation with carbon-ion microbeams and the trajectory of crawling was measured by image processing. Motility was lower after anterior-half body irradiation than after posterior-half body irradiation. This further supported that the anterior half of the body is important in the locomotory response to radiation. |
| DOI | 10.3390/ijms22189810 |
| PMID | 34575973 |