スギヤマ　ヒトシ   杉山　斉    所属   川崎医療短期大学  教育部 医療介護福祉学科    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy.
掲載誌名	正式名：Nephrology (Carlton, Vic.) 略　 称：Nephrology (Carlton) ISSNコード：14401797/13205358
掲載区分	国外
巻・号・頁	23(9),pp.855-862
著者・共著者	Tanaka Keiko, Sugiyama Hitoshi, Yamanari Toshio, Mise Koki, Morinaga Hiroshi, Kitagawa Masashi, Onishi Akifumi, Ogawa-Akiyama Ayu, Tanabe Katsuyuki, Eguchi Jun, Ohmoto Yasukazu, Shikata Kenichi, Wada Jun
発行年月	2018/09
概要	AIM:Trefoil factor 3 (TFF3) is a small peptide that is involved in mucosal protection. TFF3 is widely expressed in multiple tissues including kidney tissue. Previous studies have reported that the levels of urinary TFF3 are significantly increased in patients with chronic kidney disease. The aim of this study is to detect the TFF3 mRNA in kidney and elucidate the relationship between renal TFF3 mRNA and tubulointerstitial fibrosis in IgA nephropathy (IgAN).METHODS:We investigated the renal mRNA expression of TFF3 by real-time PCR analysis in biopsy specimens from patients with IgAN, other glomerulonephritis (OGN) and minor glomerular abnormalities (MGA). We also determined the renal localization of TFF3 and the levels of urinary TFF3 by immunostaining and ELISA, respectively.RESULTS:The renal TFF3 mRNA expression was significantly associated with the urinary TFF3 secretion and the tubulointerstitial fibrosis score in the IgAN group alone. Immunostaining of the renal specimen of IgAN patients revealed that TFF3 is located in the renal tubular epithelial cells. The locations were almost the same as those that showed uromodulin positivity; specifically, the thick ascending limb (TAL) of the loop of Henle and the early portion of the distal tubule. The urinary TFF3 levels were positively correlated with the levels of urinary biomarkers of tubulointerstitial injury in such patients.CONCLUSION:Renal TFF3 mRNA is associated with renal tubulointerstitial fibrosis in IgAN patients. The TFF3 located in the renal tubular epithelial cells may play a role in the progression of tubulointerstitial fibrosis in IgAN patients.
DOI	10.1111/nep.13444
PMID	29987860