イトウ　タツオ   Tatsuo Ito   伊藤　達男    所属   川崎医科大学  医学部 応用医学　衛生学    職種   教授
論文種別	研究論文（学術雑誌）
言語種別	英語
査読の有無	査読あり
表題	Impact of Age and Gender on Serum Cytokine and Chemokine Levels: Analysis of 157 Healthy Subjects across Consecutive Age Groups.
掲載誌名	正式名：International archives of allergy and immunology 略　 称：Int Arch Allergy Immunol ISSNコード：10182438/14230097
掲載区分	国外
巻・号・頁	pp.1-11
著者・共著者	Tasuku Miwa, Yurika Shimizu, Tatsuo Ito, Naoko Inomata, Eisuke Inoue, Hirohiko Sueki
発行年月	2026/03/09
概要	INTRODUCTION:Understanding the effects of age and sex on circulating cytokine and chemokine levels in healthy individuals is essential for establishing reliable reference ranges and tailoring assessments. Although previous studies have compared younger and older populations, few have systematically examined sequential age decades. This study aimed to characterize continuous age-related changes in serum cytokine and chemokine profiles across adulthood in 157 healthy individuals.METHODS:Serum samples from 100 individuals aged 20s-60s (10 males and 10 females per decade) were obtained from the Japanese Red Cross Society. Additional samples from 57 individuals aged 60s-80s (17-20 per decade) were included. Concentrations of 26 cytokines and chemokines were measured using a multiplex bead-based immunoassay. Associations between analyte levels and age were assessed using the Jonckheere test and Spearman's rank correlation. Sex differences were evaluated using Student's t test. A two-sided p value <0.05 was considered statistically significant.RESULTS:Three distinct age-related patterns were identified. Type 1 demonstrated continuous trends from the 20s to the 80s: interleukin (IL)-2, IP-10, thymus and activation-regulated chemokine, and granulysin increased significantly with age, whereas TGF-β, fibroblast growth factor-2, and fractalkine decreased significantly. Type 2a (interferon-γ, IL-17, and IL-15) showed significant declines from the 20s to the 60s but an overall increase from the 20s to the 80s. Type 2b included IL-12p70, tumor necrosis factor-α, IL-10, IL-5, and IL-7, which increased significantly from the 20s to the 80s, and IL-8, which decreased; however, no significant trends were observed between the 20s and 60s. Type 2c (IL-6 and IL-1Ra) increased significantly from the 20s to the 60s but showed no significant trend across the 20s-80s. Between the 20s and 60s, IL-15, IL-1Ra, TGF-β, and IP-10 were significantly higher in females, whereas IL-8 and granulysin we