コンド　マユミ   Mayumi Kondo   近土　真由美    所属   川崎医療福祉大学  医療技術学部 臨床工学科    職種   講師
論文種別	研究論文（学術雑誌）
言語種別	英語
査読の有無	査読あり
表題	Inactivation of Surface-Associated Viruses in Real Indoor Environments by a Humidification System Generating Vaporized Free Chlorine Components
掲載誌名	正式名：Microorganisms
掲載区分	国外
巻・号・頁	14(4)
総ページ数	15
著者・共著者	Saki Kawahata Mayumi Kondo Atsushi Yamada Naoya Shimazaki Makoto Saito Hiroyuki Tsukagoshi Takayoshi Takano Tetsuyoshi Yamada Toshihiro Takei akashi Nakagawa Miu Takada Nobuhiro Saruki Hirokazu Kimura
発行年月	2026/04/02
概要	Vaporized free chlorine, primarily present as hypochlorous acid (HOCl), is increasingly used for indoor microbial control; however, virus-dependent susceptibility and its molecular determinants remain unclear. We evaluated virucidal effects under controlled indoor conditions (0–9 ppb) against echovirus 30 (E30), influenza A/H1N1, and human adenovirus type 3 (HAdV3). Infectious titers were quantified by TCID50 assays. Computational fluid dynamics (CFD) simulations and gas-sensor measurements assessed spatial dispersion, and structural analyses examined oxidation-sensitive amino acid residues. Significant reductions in infectivity were observed for E30 (99.0%, p = 0.00727) and influenza A/H1N1 (99.9%, p = 0.000597), whereas no significant reduction was detected for HAdV3 (p = 0.142). Analyses including all data points without outlier exclusion confirmed the robustness of these findings. CFD indicated uniform dispersion, although spatial heterogeneity within the indoor environment cannot be excluded. These findings suggest that viral susceptibility to vaporized HOCl is associated with residue-level composition and structural context; however, this relationship should be interpreted as correlative rather than causal. Moreover, integration of molecular and structural analyses provides a plausible mechanistic framework, although direct biochemical validation remains necessary. Structural analyses showed lower proportions of oxidation-sensitive residues in adenoviral proteins compared with influenza A hemagglutinin (OR = 0.34–0.40, adjusted p < 0.001) and the E30 VP1 intermediate. Residues were clustered in surface-exposed functional domains in susceptible viruses.
researchmap用URL	https://doi.org/10.3390/microorganisms14040814