オカモト　カズコ   Kazuko Okamoto   岡本　和子    所属   川崎医科大学  医学部 一般教養　自然科学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Pressure-induced changes on the morphology and gene expression in mammalian cells.
掲載誌名	正式名：Biology open 略　 称：Biol Open ISSNコード：20466390/20466390
掲載区分	国外
巻・号・頁	(10)
著者・共著者	Kazuko Okamoto, Tomonobu M Watanabe, Masanobu Horie, Masayoshi Nishiyama, Yoshie Harada, Hideaki Fujita
担当区分	筆頭著者
発行年月	2021/07/15
概要	We evaluated the effect of high hydrostatic pressure on mouse embryonic fibroblasts (MEFs) and mouse embryonic stem (ES) cells. Hydrostatic pressures of 15, 30, 60, and 90 MPa were applied for 10 min, and changes in gene expression were evaluated. Among genes related to mechanical stimuli, death-associated protein 3 was upregulated in MEF subjected to 90 MPa pressure; however, other genes known to be upregulated by mechanical stimuli did not change significantly. Genes related to cell differentiation did not show a large change in expression. On the other hand, genes related to pluripotency, such as Oct4 and Sox2, showed a twofold increase in expression upon application of 60 MPa hydrostatic pressure for 10 min. Although these changes did not persist after overnight culture, cells that were pressurized to 15 MPa showed an increase in pluripotency genes after overnight culture. When mouse ES cells were pressurized, they also showed an increase in the expression of pluripotency genes. These results show that hydrostatic pressure activates pluripotency genes in mammalian cells. This article has an associated First Person interview with the first author of the paper.
DOI	10.1242/bio.058544
PMID	34258610