オカモト　カズコ   Kazuko Okamoto   岡本　和子    所属   川崎医科大学  医学部 一般教養　自然科学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The use of a genetically encoded molecular crowding sensor in various biological phenomena.
掲載誌名	正式名：Biophysics and physicobiology 略　 称：Biophys Physicobiol ISSNコード：21894779/21894779
掲載区分	国外
巻・号・頁	14,pp.119-125
著者・共著者	Hiroaki Machiyama, Takamitsu J Morikawa, Kazuko Okamoto, Tomonobu M Watanabe, Hideaki Fujita
発行年月	2017
概要	We evaluated usability of a previously developed genetically encoded molecular crowding sensor in various biological phenomena. Molecular crowding refers to intracellular regions that are occupied more by proteins and nucleotides than by water molecules and is thought to have a strong effect on protein function. To evaluate intracellular molecular crowding, usually the diffusion coefficient of a probe is used because it is related to mobility of the surrounding molecular crowding agents. Recently, genetically encoded molecular crowding sensors based on Förster resonance energy transfer were reported. In the present study, to evaluate the usability of a genetically encoded molecular crowding sensor, molecular crowding was monitored during several biological events. Changes in molecular crowding during stem cell differentiation, cell division, and focal adhesion development and difference in molecular crowding in filopodia locations were examined. The results show usefulness of the genetically encoded molecular crowding sensor for understanding the biological phenomena relating to molecular crowding.
DOI	10.2142/biophysico.14.0_119
PMID	28900589