マツイ　ユウト   Yuuto Matsui   松井　優人    所属   川崎医科大学  医学部 基礎医学　微生物学    職種   助教
論文種別	研究論文（学術雑誌）
言語種別	英語
査読の有無	査読あり
表題	Development of a real-time RT-PCR system applicable for rapid and pen-side diagnosis of foot-and-mouth disease using a portable device, PicoGene® PCR1100
掲載誌名	正式名：Journal of Virological Methods 略　 称：J Virol Methods.
掲載区分	国外
巻・号・頁	319(1)
国際共著	国際共著
著者・共著者	Yuto Matsui, Jeeranant Chottikamporn, Sahawatchara Ungvanijban, Kingkarn Boonsuya Seeyo, Ratchaneekorn Vitoonpong, Nutthakarn Suwankitwat, Tapanut Songkasupa, Junzo Norimine, Kentaro Yamada, Lerdchai Chintapitaksakul, Naoaki Misawa
担当区分	筆頭著者
発行年月	2023/09
概要	Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease, causing devastating losses to the livestock industry. A diagnostic method that enables quick decisions is required to control the disease, especially in FMD-free countries. Although conventional real-time reverse transcription polymerase chain reaction (RT-PCR) is a highly sensitive method widely used for the diagnosis of FMD, a time lag caused by the transport of samples to a laboratory may allow the spread of FMD. Here, we evaluated a real-time RT-PCR system using a portable PicoGene PCR1100 device for FMD diagnosis. This system could detect the synthetic FMD viral RNA within 20 min with high sensitivity compared to a conventional real-time RT-PCR. Furthermore, the Lysis Buffer S for crude nucleic extraction improved the viral RNA detection of this system in a homogenate of vesicular epithelium samples collected from FMD virus-infected animals. Furthermore, this system could detect the viral RNA in crude extracts prepared using the Lysis Buffer S from the vesicular epithelium samples homogenized using a Finger Masher tube, which allows easy homogenization without any equipment, with a high correlation compared to the standard method. Thus, the PicoGene device system can be utilized for the rapid and pen-side diagnosis of FMD.