イハラ　トシコ   Toshiko Ito-Ihara   猪原　登志子    所属   川崎医科大学  医学部 応用医学　先端医療開発学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Trafficking of QD-conjugated MPO-ANCA in murine systemic vasculitis and glomerulonephritis model mice.
掲載誌名	正式名：Microbiology and immunology 略　 称：Microbiol Immunol ISSNコード：03855600/03855600
掲載区分	国外
巻・号・頁	51(5),pp.551-66
著者・共著者	Akiyoshi Hoshino, Tomokazu Nagao, Toshiko Ito-Ihara, Akiko Ishida-Okawara, Kazuko Uno, Eri Muso, Noriko Nagi-Miura, Naohito Ohno, Kazuhiro Tokunaka, Shiro Naoe, Hiroshi Hashimoto, Masato Yasuhara, Kenji Yamamoto, Kazuo Suzuki
発行年月	2007
概要	In systemic vasculitis, the serum level of myeloperoxidase (MPO)-specific anti-neutrophil cytoplasmic autoantibodies (MPO-ANCA) is significantly elevated with the progression of disease. We have established a model of murine systemic vasculitis by administration of MPO-ANCA and fungal mannoprotein to C57BL/6 mice. We examined the role of MPO and MPO-ANCA in the pathogenesis of glomerulonephritis and systemic vasculitis in this model using quantum dots (QDs). We demonstrated that QD-conjugated MPO-ANCA (ANCA-QD) visualized the translocation of MPO on the neutrophil membrane surface after stimulation with proinflammatory cytokines. We also observed that MPO translocation on neutrophils in both patients with rapid progressive glomerulonephritis and these model mice without any stimulation, suggesting that MPO translocation is certain to contribute to the development of glomerular lesion. In addition, blood flow on the kidney surface vessel was significantly decelerated in both SCG/Kj mice and this model, suggesting that ANCA induces the damage of blood vessel. These results indicate that MPO-ANCA and surface-translocated MPO on the activated neutrophils coordinately plays essential roles in the initial steps of the glomerulonephritis.
DOI	10.1111/j.1348-0421.2007.tb03933.x
PMID	17579266