ヤマウチ　アキラ   Akira Yamauchi   山内　明    所属   川崎医科大学  医学部 基礎医学　生化学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Establishment of a method to evaluate the dynamics of highly chemotactic THP-1 cells during differentiation into monocyte-M1 macrophage-like cells
掲載誌名	正式名：In Vitro Cellular & Developmental Biology - Animal ISSNコード：10712690
掲載区分	国外
出版社	Springer Nature
著者・共著者	Shuichiro Okamoto, Kei Miyano, Yasumitsu Nishimura, Nahoko Tomonobu, Rie Kinoshita, Masakiyo Sakaguchi, Einosuke Ikeshita, Ayasa Kamezaki, Aya Morihara, Futoshi Kuribayashi, Akira Yamauchi
担当区分	責任著者
発行年月	2025
概要	Differentiation of the human monocytic leukemia cell line THP-1 is widely used to analyze the function of monocyte/macrophage-like cells in vitro. Although chemotaxis, a critical function of monocytes/macrophages enabling tissue accumulation, has been extensively studied, methods to evaluate sustained, long-distance chemotaxis remain underexplored. Therefore, we aimed to evaluate macrophage-like cells in vitro by differentiating THP-1 cells into monocyte/macrophage-like cells exhibiting sustained, strong chemotaxis over long distances (up to 260 μm). Using various reagents, we identified the combination of vitamin D, panobinostat, and granulocyte–macrophage-colony-stimulating factor as optimal for achieving high directionality and velocity in cell migration, as analyzed using the TAXIScan cell dynamics assay device. The differentiated cells matured into M1 macrophage-like cells and displayed reduced migratory capacity post-maturation, along with enhanced phagocytosis and reactive oxygen species production. Collectively, our differentiation and analysis methods provide a reliable platform for basic research into cellular maturation processes and drug development targeting the regulation of monocyte/macrophage dynamics.