ババ　ノブヤス   馬場　伸育    所属   川崎医科大学  医学部 基礎医学　免疫学    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Differential accumulation and function of proinflammatory 6-sulfo LacNAc dendritic cells in lymph node and colon of Crohn's versus ulcerative colitis patients.
掲載誌名	正式名：Journal of leukocyte biology 略　 称：J Leukoc Biol ISSNコード：19383673/07415400
掲載区分	国外
巻・号・頁	98(4),pp.671-81
著者・共著者	Marwa Bsat, Laurence Chapuy, Nobuyasu Baba, Manuel Rubio, Benoit Panzini, Ramses Wassef, Carole Richard, Genevieve Soucy, Heena Mehta, Marika Sarfati
発行年月	2015/10
概要	Human Slan DCs have been studied in patients with psoriasis, rheumatoid arthritis, cancer, and autoimmune diseases. In this study, we investigated the frequency, phenotype, and function of Slan DCs in blood, colon, as well as mLNs of patients with IBD. We first show that the frequency of circulating CD14(dull)Slan DCs was reduced in CD patients refractory to immunosuppressive drugs or TNF-α blockers relative to untreated CD, UC, and healthy subjects. In blood of CD patients, Slan DCs expressed CD172a, as detected by CD47 fusion protein binding, when compared with its lack of expression in control subjects. Next, we demonstrate that CD172a(+)Slan DCs that produced IL-1β and TNF-α accumulated in mLNs and colons of CD patients. The CD172a(+)Slan DCs up-regulated their expression of CD14 in CD tissues and the proinflammatory cytokines were produced in CD14(bright)CD172a(+)Slan DCs. By contrast, no difference was noted in the frequency of Slan DCs between inflamed, noninflamed colonic mucosa of UC patients and control, non-IBD donors. Finally, the percentage of cytokine-producing Slan DCs also augmented in response to TLR2 and NOD2 in in vitro stimulation in PBMCs of CD, but not UC, patients. In conclusion, we propose that proinflammatory CD14(bright)CD172a(+)Slan DCs are a distinguishing feature between CD and UC, as these cells accumulate uniquely in mLNs and colonic mucosa of CD patients. Thus, Slan DCs may contribute to CD immunopathogenesis.
DOI	10.1189/jlb.5A1014-509RR
PMID	26162403