イツミ　モモエ   Momoe Itsumi   逸見　百江    所属   川崎医科大学  医学部 一般教養　自然科学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Twist1 and Y-box-binding protein-1 promote malignant potential in bladder cancer cells.
掲載誌名	正式名：BJU international 略　 称：BJU Int ISSNコード：1464410X/14644096
掲載区分	国外
巻・号・頁	108(2 Pt 2),pp.E142-9
著者・共著者	Masaki Shiota, Akira Yokomizo, Momoe Itsumi, Takeshi Uchiumi, Yasuhiro Tada, YooHyun Song, Eiji Kashiwagi, Daisuke Masubuchi, Seiji Naito
発行年月	2011/07
概要	OBJECTIVE:To investigate the roles of Twist1 and Y-box binding protein-1 (YB-1) and their potential as therapeutic targets in bladder cancer (BC), as both have been suggested to play important roles in tumour growth, invasion and drug resistance.MATERIALS AND METHODS:Bladder cancer cell lines (TCCsup, UMUC3, T24 and KK47 cells) were used. Twist1 and YB-1 expression levels were assessed by luciferase reporter assay, quantitative real-time polymerase chain reaction (PCR) and western blot analysis. Tumour growth and cell cycle were analysed by cell proliferation assay and flow cytometry, respectively. Invasive and motile abilities were investigated by scratch-wound test and migration assay, respectively. Cytotoxicity assay was performed to determine drug sensitivity.RESULTS:The findings showed that Twist1 regulated YB-1 expression in BC cells. Both Twist1 and YB-1 were involved in cell growth, invasion, motility and resistance to cisplatin and doxorubicin, but not to 5-fluorouracil (5-FU).CONCLUSION:The present study showed that Twist1 regulates YB-1 expression and that both Twist1 and YB-1 promote malignant potentials, including tumour growth, invasion and anti-cancer-drug resistance, indicating that both Twist1 and YB-1 are novel molecular targets in BC.
DOI	10.1111/j.1464-410X.2010.09810.x
PMID	21083641