イツミ　モモエ   逸見　百江    所属   川崎医科大学  医学部 一般教養　自然科学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Dual specificity phosphatase16 is a negative regulator of c-Jun NH2-terminal kinase activity in T cells.
掲載誌名	正式名：Microbiology and immunology 略　 称：Microbiol Immunol ISSNコード：03855600/03855600
掲載区分	国外
巻・号・頁	54(2),pp.105-11
著者・共著者	Shino Kumabe, Momoe Itsumi, Hisakata Yamada, Toshiki Yajima, Tetsuya Matsuguchi, Yasunobu Yoshikai
発行年月	2010/02
概要	Activation of MAPK is negatively regulated by DUSP, which dephosphorylate the phosphothreonine and phosphotyrosine residues. We have identified a novel JNK-specific DUSP, DUSP16, from murine macrophages. Its involvement in T cells has not yet been defined. In the present study, we found expression of DUSP16 in thymocytes and activated T cells but not in naive T cells. To elucidate the roles of DUSP16 in T cells, transgenic mice expressing a dominant negative form of DUSP16 specifically in T cells were generated (dnDUSP16 Tg). JNK activity was selectively augmented in the thymocytes of these dnDUSP16 Tg mice. CD4 T cells in dnDUSP16 Tg mice showed normal levels of proliferation and IL-2 production after TCR triggering, while they produced increased IFN-gamma but reduced Th2 cytokines compared with wild type CD4 T cells. On the other hand CD8 T cells in dnDUSP16 Tg mice produced an increased amount of IL-2, which resulted in enhanced proliferation and IFN-gamma production. These results suggest that DUSP16 is an important regulator of JNK activity and effector functions of CD4 and CD8 T cells.
DOI	10.1111/j.1348-0421.2009.00188.x
PMID	20377744