ミウラ　マズミ   Mazumi Miura   三浦　真澄    所属   川崎医科大学  医学部 臨床医学　小児科学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Tear fluid cytokine analysis: a non-invasive approach for assessing retinopathy of prematurity severity.
掲載誌名	正式名：Japanese journal of ophthalmology 略　 称：Jpn J Ophthalmol ISSNコード：16132246/00215155
掲載区分	国外
著者・共著者	Takashi Baba, Ryu Uotani, Kodai Inata, Shin-Ichi Sasaki, Yumiko Shimizu, Mazumi Miura, Yoshitsugu Inoue, Dai Miyazaki
発行年月	2024/07
概要	PURPOSE:To determine whether there is a significant association between inflammatory cytokines in the tear fluid and the severity of Retinopathy of Prematurity (ROP).STUDY DESIGN:Retrospective cohort study.METHODS:The cytokine levels in tear fluids were determined in 34 eyes with ROP and 18 eyes without ROP. There were 15 eyes with severe ROP requiring treatment and 19 eyes with mild ROP not requiring treatment. For severe ROP eyes, tear fluids were collected before treatment.RESULTS:Significantly higher levels of CCL2 and vascular endothelial growth factor (VEGF) were detected in eyes with severe ROP compared to eyes with mild ROP and no ROP. When assessed for cytokine levels that discriminate each disease group, CCL2 showed a significant odds ratio of 1.76 for severity change (/quintile, P = 0.032, after adjusting for birth weight). Correlation analysis showed that birth weight correlated with IL-1α levels, and decreased weight gain increased IFN-γ levels. We next determined tear fluid cytokines which discriminate severe ROP using receiver operating characteristics' analysis. We found that combination of higher CCL2 levels, higher VEGF levels, and lower IFN-γ levels in the tear fluid had a stronger predictive value for severe ROP (area under curve, 0.85).CONCLUSION:The levels of CCL2, VEGF, and IFN-γ in tear fluid may serve as useful biomarkers for assessing the severity of ROP.
DOI	10.1007/s10384-024-01084-0
PMID	38985404