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ミウラ マズミ
Mazumi Miura
三浦 真澄 所属 川崎医科大学 医学部 臨床医学 小児科学 職種 教授 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Increased IRS2 mRNA Expression in SGA Neonates: PCR Analysis of Insulin/IGF Signaling in Cord Blood. |
| 掲載誌名 | 正式名：Journal of the Endocrine Society |
| 掲載区分 | 国外 |
| 巻・号・頁 | 1(12),pp.1408-1416 |
| 著者・共著者 | Masanobu Fujimoto,Yuki Kawashima Sonoyama,Kenji Fukushima,Aya Imamoto,Fumiko Miyahara,Naoki Miyahara,Rei Nishimura,Yuko Yamada,Mazumi Miura,Kaori Adachi,Eiji Nanba,Keiichi Hanaki,Susumu Kanzaki |
| 発行年月 | 2017/12/01 |
| 概要 | Context: Hypoglycemia is the most common metabolic problem among small-for-gestational-age (SGA) neonates. However, the pathological mechanism and insulin/ insulin-like growth factor (IGF) signaling axis in neonates remain unknown. Objective: To determine the insulin/IGF axis in neonates, we analyzed the messenger RNA (mRNA) expression of insulin/IGF signaling in fetal umbilical cord blood. Setting: The Perinatal Medical Center of Tottori University Hospital. Participants: Fifty-two [42 appropriate-for-gestational-age (AGA) and 10 SGA] neonates. Interventions: Immediately collected cord blood was placed into a PAXgene Blood RNA Tube. Total RNA from the blood was purified using reagents provided in the PAXgene Blood RNA Kit within 4 days, and reverse transcription polymerase chain reaction (PCR) was performed. Main Outcome Measure: Quantitative real-time PCR analysis was applied to evaluate the mRNA expression of insulin receptor (INSR), IGF-I receptor (IGF1R), insulin receptor substrate 1 (IRS1), IRS2, and glucose transporters (SLC2A2 and SLC2A4). β-Actin was used as a control gene. Results: Serum glucose and IGF-I levels in SGA neonates were significantly lower. The cord serum insulin levels were similar between AGA and SGA neonates. The IRS2 mRNA expression was significantly higher in SGA than in AGA neonates (P < 0.05). The IRS2 mRNA expression was significantly higher in hypoglycemic SGA neonates than in normoglycemic SGA neonates. Conclusions: We determined that intrauterine growth restriction induces increased IRS2 mRNA expression in cord blood, without hyperinsulinemia. The increased expression of IRS2 mRNA might be associated with abnormal glucose metabolism in SGA neonates. Our findings might lead to the elucidation of abnormal glucose metabolism in SGA neonates. |
| DOI | 10.1210/js.2017-00294 |
| PMID | 29264464 |