ツボイ　カズヒト   Kazuhito Tsuboi   坪井　一人    所属   川崎医科大学  医学部 基礎医学　薬理学    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Lysophospholipase D activity on oral mucosa cells in whole mixed human saliva involves in production of bioactive lysophosphatidic acid from lysophosphatidylcholine.
掲載誌名	正式名：Prostaglandins & Other Lipid Mediators 略　 称：Prostaglandins Other Lipid Mediat ISSNコード：10988823/10988823
掲載区分	国外
巻・号・頁	174,pp.106881
著者・共著者	Tsutsumi T, Taira S, Matsuda R, Kageyama C, Wada M, Kitayama T, Morioka N, Morita K, Tsuboi K, Yamazaki N, Kido J, Nagata T, Dohi T, Tokumura A
発行年月	2024/08/10
概要	We reported that lysophosphatidic acid (LPA) is present at 0.8 μM in mixed human saliva (MS). In this study, we examined the distribution, origin, and enzymatic generation pathways of LPA in MS. LPA was distributed in the medium and cell pellet fraction; a true level of soluble LPA in MS was about 150 nM. The soluble LPA was assumed to be generated by ecto-type lysophospholipase D on exfoliated cells in MS from LPC that originated mainly from the major salivary gland saliva. Our results with the albumin-back extraction procedures suggest that a significant pool of LPA is kept in the outer layer of the plasma membranes of detached oral mucosal cells. Such pool of LPA may contribute to wound healing in upper digestive organs including oral cavity. We obtained evidence that the choline-producing activity in MS was mainly due to Ca2+-activated lysophospholipase D activity of glycerophosphodiesterase 7.
DOI	10.1016/j.prostaglandins.2024.106881
PMID	39134206