ヤマウチ　アキラ   Akira Yamauchi   山内　明    所属   川崎医科大学  医学部 基礎医学　生化学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Lysyl oxidase-like 4 promotes the invasiveness of triple-negative breast cancer cells by orchestrating the invasive machinery formed by annexin A2 and S100A11 on the cell surface
掲載誌名	正式名：Frontiers in Oncology 略　 称：Front Oncol. ISSNコード：2234943X
掲載区分	国外
出版社	Frontiers Media S.A.
巻・号・頁	14
総ページ数	12
国際共著	国際共著
著者・共著者	Tetta Takahashi,Nahoko Tomonobu,Rie Kinoshita,Ken-ichi Yamamoto,Hitoshi Murata,Ni Luh Gede Yoni Komalasari,Youyi Chen,Fan Jiang,Yuma Gohara,Toshiki Ochi,I Made Winarsa Ruma,I Wayan Sumardika,Jin Zhou,Tomoko Honjo,Yoshihiko Sakaguchi,Akira Yamauchi,Futoshi Kuribayashi,Eisaku Kondo,Yusuke Inoue,Junichiro Futami,Shinichi Toyooka,Yoshito Zamami,Masakiyo Sakaguchi
発行年月	2024/03/26
概要	Background: LOX family members are reported to play pivotal roles in cancer. Unlike their enzymatic activities in collagen cross-linking, their precise cancer functions are unclear. We revealed that LOXL4 is highly upregulated in breast cancer cells, and we thus sought to define an unidentified role of LOXL4 in breast cancer. Methods: We established the MDA-MB-231 sublines MDA-MB-231-LOXL4 mutCA and -LOXL4 KO, which stably overexpress mutant LOXL4 that loses its catalytic activity and genetically ablates the intrinsic LOXL4 gene, respectively. In vitro and in vivo evaluations of these cells' activities of cancer outgrowth were conducted by cell-based assays in cultures and an orthotopic xenograft model, respectively. The new target (s) of LOXL4 were explored by the MS/MS analytic approach. Results: Our in vitro results revealed that both the overexpression of mutCA and the KO of LOXL4 in cells resulted in a marked reduction of cell growth and invasion. Interestingly, the lowered cellular activities observed in the engineered cells were also reflected in the mouse model. We identified a novel binding partner of LOXL4, i.e., annexin A2. LOXL4 catalyzes cell surface annexin A2 to achieve a cross-linked multimerization of annexin A2, which in turn prevents the internalization of integrin β-1, resulting in the locking of integrin β-1 on the cell surface. These events enhance the promotion of cancer cell outgrowth. Conclusions: LOXL4 has a new role in breast cancer progression that occurs via an interaction with annexin A2 and integrin β-1 on the cell surface.
DOI	10.3389/fonc.2024.1371342