クロダ　コウスケ   Kosuke Kuroda   黒田　浩佐    所属   川崎医科大学  医学部 臨床医学　麻酔・集中治療医学    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Induction of hepatic Bach1 mRNA expression by carbon tetrachloride-induced acute liver injury in rats.
掲載誌名	正式名：Biomedical reports 略　 称：Biomed Rep ISSNコード：20499434/20499434
掲載区分	国外
巻・号・頁	2(3),pp.359-363
著者・共著者	Nohito Tanioka, Hiroko Shimizu, Toru Takahashi, Emiko Omori, Kosuke Kuroda, Mari Shibata, Masakazu Yamaoka, Yuichiro Toda, Takashi Matsusaki, Hiroshi Morimatsu
発行年月	2014/05
概要	Hepatic oxidative stress is a major contributor to the pathogenesis of several acute liver diseases. Diagnostic markers of hepatic oxidative stress may facilitate early detection and intervention. Bach1 is an oxidative stress-responsive transcription factor that represses heme oxygenase 1 (HO-1), the rate-limiting enzyme in the catabolism of heme, a potent pro-oxidant. We previously demonstrated that carbon tetrachloride (CCl4) causes oxidative hepatic injury in rats, exacerbated by free heme, suggesting that CCl4 may affect Bach1 gene expression. In the present study, we used northern blot analysis to measure Bach1, HO-1 and δ-aminolevulinate synthase (ALAS1; a heme biosynthesis enzyme) mRNA expression levels during acute hepatic injury induced by CCl4 (at doses of 0.1, 1.0 and 2.0 ml/kg body weight). Oxidative injury was assessed by measuring serum alanine aminotransferase (ALT), hepatic malondialdehyde (MDA) and glutathione (GSH) content. Treatment with CCl4 induced a significant dose-dependent increase in Bach1 mRNA 1-3 h after administration. Bach1 mRNA peaked at 6 h after CCl4 treatment (1 ml/kg), followed by a rapid decrease and gradual return to baseline by 12 h after treatment. The timecourse of transient Bach1 mRNA induction roughly mirrored that of HO-1 mRNA, while ALAS1 mRNA was inversely downregulated. Serum ALT levels and hepatic MDA concentration were significantly increased at 24 h after CCl4 treatment, while the hepatic GSH content was significantly reduced within 3 h of treatment. Serum ALT levels were positively correlated with Bach1 mRNA levels. These findings indicate that Bach1 mRNA is transiently induced in rat liver by CCl4, possibly as a regulatory mechanism to restore HO-1 to baseline following free heme catabolism. Our findings also suggest that Bach1 mRNA expression may be a novel indicator of the extent of oxidative hepatic injury caused by free heme.
DOI	10.3892/br.2014.235
PMID	24748974