ヤマニシ　ハルヨ   Haruyo Yamanishi   山西　治代    所属   川崎医科大学  医学部 基礎医学　解剖学    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation.
掲載誌名	正式名：Scientific reports ISSNコード：20452322
掲載区分	国外
巻・号・頁	9,pp.12601-253
著者・共著者	Egawa M, Iwanaga S, Hosoi J, Goto M, Yamanishi H, Miyai M, Katagiri C, Tokunaga K, Asai T, Ozeki Y
発行年月	2019/08
概要	Epidermal keratinocyte (KC) differentiation, which involves the process from proliferation to cell death for shedding the outermost layer of skin, is crucial for the barrier function of skin. Therefore, in dermatology, it is important to elucidate the epidermal KC differentiation process to evaluate the symptom level of diseases and skin conditions. Previous dermatological studies used staining or labelling techniques for this purpose, but they have technological limitations for revealing the entire process of epidermal KC differentiation, especially when applied to humans. Here, we demonstrate label-free visualization of three-dimensional (3D) intracellular morphological changes of ex vivo human epidermis during epidermal KC differentiation using stimulated Raman scattering (SRS) microscopy. Specifically, we observed changes in nuclei during the initial enucleation process in which the nucleus is digested prior to flattening. Furthermore, we found holes left behind by improperly digested nuclei in the stratum corneum, suggesting abnormal differentiation. Our findings indicate the great potential of SRS microscopy for discrimination of the degree of epidermal KC differentiation.