モリヤ　タクヤ   Takuya Moriya   森谷　卓也    所属   川崎医科大学  医学部    職種   学長付特任教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The Urinary Bladder is Rich in Glycosphingolipids Composed of Phytoceramides.
掲載誌名	正式名：Journal of lipid research 略　 称：J Lipid Res ISSNコード：15397262/00222275
掲載区分	国外
巻・号・頁	63(12),pp.100303
著者・共著者	Takashi Watanabe, Akemi Suzuki, Shin Ohira, Shinji Go, Yuta Ishizuka, Takuya Moriya, Yoshiyuki Miyaji, Tota Nakatsuka, Keita Hirata, Atsushi Nagai, Junko Matsuda
発行年月	2022/12
概要	Glycosphingolipids (GSLs) are composed of a polar glycan chain and a hydrophobic tail known as ceramide. Together with variation in the glycan chain, ceramides exhibit tissue-specific structural variation in the long-chain base (LCB) and N-acyl chain moieties in terms of carbon chain length, degree of desaturation, and hydroxylation. Here, we report the structural variation in GSLs in the urinary bladders of mice and humans. Using TLC, we showed that the major GSLs are hexosylceramide, lactosylceramide, globotriaosylceramide, globotetraosylceramide, Neu5Ac-Gal-Glc-Ceramide, and Neu5Ac-Neu5Ac-Gal-Glc-Ceramide. Our LC-MS analysis indicated that phytoceramide structures with a 20-carbon LCB (4-hydroxyeicosasphinganine) and 2-hydroxy fatty acids are abundant in hexosylceramide and Neu5Ac-Gal-Glc-Ceramide in mice and humans. In addition, quantitative PCR demonstrated that DES2 and FA2H, which are responsible for the generation of 4-hydroxysphinganine and 2-hydroxy fatty acid, respectively, and SPTLC3 and SPTSSB, which are responsible for the generation of 20-carbon LCBs, showed significant expressions in the epithelial layer than in the subepithelial layer. Immunohistochemically, dihydroceramide:sphinganine C4-hydroxylase (DES2) was expressed exclusively in urothelial cells of the urinary bladder. Our findings suggest that these ceramide structures have an impact on membrane properties of the stretching and shrinking in transitional urothelial cells.
DOI	10.1016/j.jlr.2022.100303
PMID	36441023