サカモト　ユウマ   Yuma Sakamoto   坂本　祐真    所属   川崎医科大学  医学部 基礎医学　免疫学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff's reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section.
掲載誌名	正式名：Biotechnic & histochemistry : official publication of the Biological Stain Commission 略　 称：Biotech Histochem ISSNコード：14737760/10520295
掲載区分	国外
巻・号・頁	98(1),pp.54-61
著者・共著者	Hiroshi Takase, Takayuki Murase, Daisuke Hachisuka, Yuma Sakamoto, Mariko Sugiura, Satsuki Nakano, Keiichiro Fujii, Ayako Masaki, Hiroshi Inagaki
発行年月	2023/01
概要	An aqueous 7-amino-4-methylcoumarin (AMC) solution exhibits strong fluorescence under ultraviolet (UV) light and can be used as a Schiff reagent to visualize aldehydes. We investigated hemalum and eosin (H & E) and AMC staining for histological and pathological analysis. Sections of normal and lesioned human tissues were stained with combined H & E/AMC staining. After H & E/AMC staining, the H & E morphology was preserved under bright field microscopy. The AMC fluorescent signals observed under UV light were intense and the staining pattern was identical to that obtained by periodic acid-Schiff (PAS) staining. AMC staining of archived H & E sections also was successful. Diastase digestion differentiated glycogen from other AMC positive elements. Using H & E/AMC staining, mucus-rich adenocarcinoma cells, amebic trophozoites and fungal hyphae were visualized clearly under UV excitation. Using H & E/AMC staining, H & E and PAS-like histological imaging can be obtained using a single tissue section. H & E/AMC is useful for pathologic diagnosis especially when information from PAS staining is critical, the number of tissue sections is limited and/or the lesion in question is small.
DOI	10.1080/10520295.2022.2101144
PMID	35904399