ミウラ　キヨシ   Kiyoshi Miura   三浦　紀称嗣    所属   川崎医療福祉大学  医療技術学部 臨床栄養学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Measurement of changes in CO2 generated from propionate in Caenorhabditis elegans using novel 13CO2 gas analysis
掲載誌名	正式名：Bioscience, Biotechnology, and Biochemistry
掲載区分	国外
出版社	Bioscience, Biotechnology, and Biochemistry
巻・号・頁	86(7),pp.884-894
総ページ数	11
著者・共著者	Kiyoshi Miura, Hiroki Yamaguchi, Keita Miyata, Tomihiro Miyada
担当区分	筆頭著者,責任著者
発行年月	2022/04
概要	Caenorhabditis elegans contains two pathways for propionate metabolism, the vitamin B12-dependent and shunt pathways, which are similar to those in humans. In this study, we monitored the changes in propionate metabolism in the whole body of C. elegans using novel 13CO2 gas analysis. We measured the increase in 13CO2 excreted in the air after administering [1-13C]-labeled propionate ([13C]propionate) to the worms. The 13CO2 generated from [13C]propionate in C. elegans increased in a dose-dependent manner, reaching a maximum at 48 h. Enhanced expression of propionate metabolism-related genes was observed after administration of propionate. Knockdown of mmcm-1, which encodes the rate-limiting enzyme of the propionate metabolism, using RNAi reduced 13CO2 excretion. Thus, 13CO2 gas analysis could be confirmed 13CO2 excretion associated with changes in the metabolism of [13C]propionate in nematodes. This analysis can contribute to further understanding of the physiological effects of short-chain fatty acids.