モリヤ　タクヤ   Takuya Moriya   森谷　卓也    所属   川崎医科大学  医学部 臨床医学　病理学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements.
掲載誌名	正式名：Scientific reports 略　 称：Sci Rep ISSNコード：20452322/20452322
掲載区分	国外
巻・号・頁	12(1),pp.8520
著者・共著者	Ueo Hiroki, Minoura Itsushi, Ueo Hiroaki, Gamachi Ayako, Kai Yuichiro, Kubota Yoko, Doi Takako, Yamaguchi Miki, Yamashita Toshinari, Tsuda Hitoshi, Moriya Takuya, Yamaguchi Rin, Kozuka Yuji, Sasaki Takeshi, Masuda Takaaki, Urano Yasuteru, Mori Masaki, Mimori Koshi
発行年月	2022/05
概要	Breast-conserving surgery has become the preferred treatment method for breast cancer. Surgical margin assessment is performed during surgery, as it can reduce local recurrence in the preserved breast. Development of reliable and lower-cost ex vivo cancer detection methods would offer several benefits for patient care. Here, a practical and quantitative evaluation method for the ex vivo fluorescent diagnosis of breast lesions was developed and confirmed through a three-step clinical study. Gamma-glutamyl-hydroxymethyl rhodamine green (gGlu-HMRG) has been reported to generate fluorescence in breast lesions. Using this probe, we constructed a reliable and reproducible procedure for the quantitative evaluation of fluorescence levels. We evaluated the reliability of the method by considering reproducibility, temperature sensitivity, and the effects of other clinicopathological factors. The results suggest that the fluorescence increase of gGlu-HMRG is a good indicator of the malignancy of breast lesions. However, the distributions overlapped. A 5 min reaction with this probe could be used to distinguish at least part of the normal breast tissue. This method did not affect the final pathological examination. In summary, our results indicate that the methods developed in this study may serve as a feasible intraoperative negative-margin assessment tool during breast-conserving surgery.
DOI	10.1038/s41598-022-12614-6
PMID	35595810