タナカ　シンイチ   Shinichi Tanaka   田中　慎一    所属   川崎医療福祉大学  医療技術学部 臨床検査学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Bright-field HER2 dual in situ hybridization (DISH) assay on breast cancer cell blocks: a comparative study with histological sections
掲載誌名	正式名：Breast Cancer
掲載区分	国外
巻・号・頁	23(6),pp.917-921
著者・共著者	Nishimura Rieko，Okamoto Nami，Satou Masakazu，Kojima Kenta， Tanaka Shinichi， Yamashita Natsumi
発行年月	2016/11/23
概要	Background: HER2 testing for samples from recurrent or metastatic disease is recommended by the 2013 update of the American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guidelines and cytological analysis can be applied to several types of metastatic lesions. However, the practical method to assess the HER2 testing of breast cancer cytology specimens has yet to be resolved. Therefore, we conducted the bright-field HER2 dual in situ hybridization (DISH) assay on cell blocks (CBs) prepared from breast cancer cell samples as a validation study before clinical use. Methods: CBs were prepared from tumor cell samples collected from 54 surgically excised breast tumors. The cells were fixed in 10 % buffered formalin for 16-28 h, and embedded in paraffin. The INFORM HER2/neu Dual ISH DNA Probe Cocktail was used for the DISH assay on the Ventana BenchMark ULTRA (Roche Diagnostics). Results: Successful results were obtained in 51 of 54 CB specimens, and the results from the CB specimens were in agreement with those from the histological sections in 48 of the 51 cases (concordance rate, 94 %; kappa, 0.846). The intraclass correlation coefficient (ICC) between the CB and histological specimens in the continuous HER2/CEP17 signal count ratio was 0.89 (95 % CI 0.81-0.93), and the Pearson's CC was 0.91 (95 % CI 0.85-0.94). Conclusion: The HER2 DISH assay, utilizing 10 % buffered formalin-fixed CB, would be a reliable and ideal method to assess the HER2 gene status of breast cancer cytological specimens. Keywords: Breast cancer; Cell block; Cytology; DISH; HER2 gene.