ハヤシ　シュウイチ   Shuichi Hayashi   林　周一    所属   川崎医科大学  医学部 基礎医学　解剖学    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Subset of cortical layer 6b neurons selectively innervates higher order nuclei in the thalamus in mouse.
掲載誌名	正式名：Cerebral Cortex
掲載区分	国外
巻・号・頁	28(5),pp.1882-1897
著者・共著者	Hoerder-Suabedissen, A., Hayashi, S., Upton, L., Nolan, Z., Casas-Torremocha, D., Grant., E., Kanold, P., Clasca, F., Kim, Y., Molnar, Z.
担当区分	2nd著者
発行年月	2018
概要	The thalamus receives input from 3 distinct cortical layers, but input from only 2 of these has been well characterized. We therefore investigated whether the third input, derived from layer 6b, is more similar to the projections from layer 6a or layer 5. We studied the projections of a restricted population of deep layer 6 cells ("layer 6b cells") taking advantage of the transgenic mouse Tg(Drd1a-cre)FK164Gsat/Mmucd (Drd1a-Cre), that selectively expresses Cre-recombinase in a subpopulation of layer 6b neurons across the entire cortical mantle. At P8, 18% of layer 6b neurons are labeled with Drd1a-Cre::tdTomato in somatosensory cortex (SS), and some co-express known layer 6b markers. Using Cre-dependent viral tracing, we identified topographical projections to higher order thalamic nuclei. VGluT1+ synapses formed by labeled layer 6b projections were found in posterior thalamic nucleus (Po) but not in the (pre)thalamic reticular nucleus (TRN). The lack of TRN collaterals was confirmed with single-cell tracing from SS. Transmission electron microscopy comparison of terminal varicosities from layer 5 and layer 6b axons in Po showed that L6b varicosities are markedly smaller and simpler than the majority from L5. Our results suggest that L6b projections to the thalamus are distinct from both L5 and L6a projections.