ソノダ　ユウジ   Yuji Sonoda   園田　祐治    所属   川崎医療福祉大学  医療福祉マネジメント学部 医療福祉デザイン学科    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Effects of colchicine on the enucleation of erythroid cells and macrophages in the liver of mouse embryos : Ultrastuctural and three-dimensional studies.
掲載誌名	正式名：The Anatomical Record
巻・号・頁	251(3),pp.290-296
著者・共著者	Sonoda Yuji, Sasaki Kazunobu, Suda Masumi, Itano Chikako, Iwatsuki Hirohiko
担当区分	筆頭著者
発行年月	1998
概要	Our aim was to clarify the significance of the enucleation in liver erythropoiesis and macrophage phagocytosis by colchicine and cytochalasin administration to embryonic mice. Pregnant mice were intraperitoneally injected with colchicine or cytochalasin at 13 days of gestation. Embryonic livers were removed at intervals of 3, 6 and 12 h after injection for processing for light and electron microscopy, and, to obtain three-dimensional morphology of erythroids at enucleation, computer-aided reconstructions were performed by light microscopy. Colchicine injections had cytolytic effects on hepatocytes and macrophages, and numerous erythroblasts were observed in the process of enucleation after colchicine injection. However, the extruding nuclei were irregularly shaped, and some erythroblasts at mitosis showed extreme peripheralization of their chromosomal masses and cell membrane constriction. Enucleation behavior could also be observed in immature erythroblasts. Liver macrophages engulfed extruded nuclei and erythroblasts in mitosis. Cytochalasin injections, on the other hand, had no significant effect on embryonic livers. The progress of erythroblast mitosis was clearly stopped by colchicine injection, and numerous erythroblasts at mitosis were extruding their nuclear compartment. Following colchicine injection, erythroid enucleation also took place in immature erythroblasts, and mitotic erythroids were phagocytosed. In enucleation, more attention should be paid to hematopoietic environmental factors than to hemopoietic cell factors.
文献番号	9669755/PubMedID