Faculty Information - Yasuo Okamoto

Present specialized field

BooK

Journal

Presentation

（Last updated : 2025-08-09 09:57:31）

Yasuo Okamoto
Department Kawasaki Medical School Kawasaki Medical School, Department of Pharmacology,

Position Professor

■ Present specialized field

Pathological biochemistry, Medical biochemistry, Pharmacology

■ BooK

2015/10

Vascular Endothelial S1P, Bioactive Lipid Mediators

■ Journal

1.	2024/07/22	GPR176 promotes fibroblast-to-myofibroblast transition in organ fibrosis progression
2.	2024/03/22	Sweat protects against contact hypersensitivity: Transient sweat suppression compromises skin barrier function in mice
3.	2023/06/20	Genetic ablation of Saposin-D in Krabbe disease eliminates psychosine accumulation but does not significantly improve demyelination
4.	2023/05/16	GDE7 produces cyclic phosphatidic acid in the ER lumen functioning as a lysophospholipid mediator.
5.	2021/10/18	Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7.
6.	2021/09/28	Sphingosine 1-phosphate receptor type 2 positively regulates interleukin (IL)-4/IL-13-induced STAT6 phosphorylation.
7.	2021/09/20	ATF4-mediated transcriptional regulation protects against β-cell loss during endoplasmic reticulum stress in a mouse model.
8.	2021/08/29	Inhibition of acid ceramidase elicits mitochondrial dysfunction and oxidative stress in pancreatic cancer cells.
9.	2021/05/24	Involvement of acid ceramidase in the degradation of bioactive N-acylethanolamines.
10.	2020/06	Growth differentiation factor 15 facilitates lung fibrosis by activating macrophages and fibroblasts.
11.	2019/09	Chronic treatment with alpha-lipoic acid improves endothelium-dependent vasorelaxation of aortas in high-fat diet-fed mice.
12.	2019/01	Class II PI3Ks alpha and beta Are Required for Rho-Dependent Uterine Smooth Muscle Contraction and Parturition in Mice
13.	2018/10	Endocannabinoids and related N-acylethanolamines: biological activities and metabolism.
14.	2018	Sphingosine-1-phosphate receptor-2 facilitates pulmonary fibrosis through potentiating IL-13 pathway in macrophages.
15.	2017	Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6.
16.	2016	Sphingosine-1-phosphate receptor type 2 (S1P2) inhibits bleomycininduced cellular senescence in murine lung fibroblasts
17.	2015	Phosphatidylinositol 3-kinase class II alpha-isoform PI3K-C2 alpha is required for transforming growth factor beta-induced Smad signaling in endothelial cells.
18.	2014	Selenoprotein P as a diabetes-associated hepatokine that impairs angiogenesis by inducing VEGF resistance in vascular endothelial cells.
19.	2013	Essential role of class II PI3K-C2 alpha in sphingosine 1-phosphate receptor-1 mediated signaling and migration in endothelial cells.
20.	2013	Sphingoshine-1-phosphate receptor 2 protects anaphylactic shock through suppression of endothelial nitric oxide synthase in mice.
21.	2013	Sphingosine-1-phosphate as a mediator involved in development of fibrotic diseases.
22.	2013	Sphingosine-1-Phosphate Signaling and Cardiac Fibrosis.
23.	2012	Antagonism of sphingosine 1-phosphate receptor 2 causes a selective reduction of portal vein pressure in bile duct-ligated rodents.
24.	2012	Endothelial PI3K-C2 alpha, a class II PI3K, has an essential role in angiogenesis and vascular barrier function.
25.	2012	Sphingosine-1-phosphate signaling in physiology and diseases.
26.	2011	Crucial role of membrane type 1 matrix metalloproteinase (MT1-MMP) in RhoA/Rac1-dependent signaling pathways in thrombin-stimulated endothelial cells.
27.	2011	Enzymatic formation of N-acylethanolamines from N-acylethanolamine plasmalogen through N-acylphosphatidylethanolamine-hydrolyzing phospholipase D-dependent and -independent pathways.
28.	2011	Intraradial administration of fasudil inhibits augmented Rho kinase activity to effectively dilate the spastic radial artery during coronary artery bypass grafting surgery.
29.	2010	Enhanced Ca²⁺-dependent activation of phosphoinositide 3-kinase class Ii alfa isoform-Rho axis in blood vessels of spontaneously hypertensive rats.
30.	2010	S1P₂, the G protein-coupled receptor for sphingosine-1-phosphate, negatively regulates tumor angiogenesis and tumor growth in vivo in mice.
31.	2010	S1P₃-mediated cardiac fibrosis in sphingosine kinase 1 transgenic mice involves reactive oxygen species.
32.	2010	Sustained delivery of sphingosine-1-phosphate using poly(lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice.
33.	2009/04	The tumor suppressor gene H-Rev107 functions as a novel Ca²⁺-independent cytosolic phospholipase A_1/2 of the thiol hydrolase type.
34.	2009	cDNA cloning and characterization of human and mouse Ca²⁺-independent phosphatidylethanolamine N-acyltransferases.
35.	2009	Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel-coated polyester stent graft.
36.	2009	Inhibitory effect of N-palmitoylphosphatidylethanolamine on macrophage phagocytosis through inhibition of Rac1 and Cdc42.
37.	2009	LOX-1-MT1-MMP axis is crucial for RhoA and Rac1 activation induced by oxidized low-density lipoprotein in endothelial cells.
38.	2008/01	The stimulatory effect of phosphatidylethanolamine on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD).
39.	2008	G_12/13 and G_q mediates S1P₂-induced inhibition of Rac and migration in vascular smooth muscle in a manner dependent on Rho but not Rho kinase.
40.	2008	Mechanisms for recycling and biosynthesis of endogenous cannabinoids anandamide and 2-arachidonylglycerol.
41.	2008	Membrane microdomains and metabolic pathways that define anandamide and 2-arachidonyl glycerol biosynthesis and breakdown.
42.	2008	The expression of osteopontin is increased in vessels with blood-brain barrier impairment.
43.	2007/11	Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism.
44.	2007/06	Discovery and characterization of a Ca²⁺-independent phosphatidylethanolamine N-acyltransferase generating the anandamide precursor and its congeners.
45.	2007/05	Predominant expression of lysosomal N-acyletanolamine-hydrolyzing acid amidase in macrophages revealed by immunochemical studies.
46.	2007	Accumulation of triosephosphate isomerase, with sequence homology to beta amyloid peptides, in vessel walls of the newborn piglet hippocampus.
47.	2006/05	Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-beta-lactamase family.
48.	2005/03	Molecular characterization of N-acylethanolamine-hydrolyzing acid amidase, a novel member of the choloylglycine hydrolase family with structural and functional similarity to acid ceramidase.
49.	2005	Distinct roles for de novo versus hydrolytic pathways of sphingolipid biosynthesis in Saccharomyces cerevisiae.
50.	2005	Involvement of N-acylethanolamine-hydrolyzing acid amidase in the degradation of anandamide and other N-acylethanolamines in macrophages.
51.	2005	Mammalian cells stably overexpressing N-acylphosphatidylethanolamine-hydrolyzing phospholipase D exhibit significantly decreased levels of N-acylphosphatidylethanolamines.
52.	2005	N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation.
53.	2005	Regional distribution and age-dependent expression of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D in rat brain.
54.	2005	Stimulation and validation of modeled sphingolipid metabolism in Saccharomyces cerevisiae .
55.	2005	The phosphatidylglycerol/cardiolipin biosynthetic pathway is required for the activation of inositol phosphosphingolipid phospholipase C, Isc1p, during growth of Saccharomyces cerevisiae .
56.	2004	Activation and localization of Inositol phosphosphingolipid phospholipase C, Isc1p, to the mitochondria during growth of Saccharomyces cerevisiae.
57.	2004	Biosynthesis of anandamide and N-palmitoylethanolamine by sequential actions of phospholipase A₂ and lysophospholipase D.
58.	2004	Evaluation of bioactive saponins and triterpenoidal aglycons for their binding properties on human endothelin ETA and angiotensin AT1 receptors.
59.	2004	Molecular Characterization of a Phospholipase D Generating Anandamide and Its Congeners.
60.	2003	Functional analysis of ISC1 by site-directed mutagenesis.
61.	2003	Roles for sphingolipid biosynthesis in mediation of specific programs of the heat stress response determined through gene expression profiling.
62.	2002	Bcl-xL interrupts oxidative activation of neutral sphingomyelinase.
63.	2002	Ca²⁺ entry channels involved in contractions of rat aorta by endothelin-1, noradrenaline, and vasopressin.
64.	2002	Inhibition of tumor necrosis factor-induced cell death in MCF7 by a novel inhibitor of neutral sphingomyelinase.
65.	2002	Molecular mechanism for endothelin-1-induced stress-fiber formation: Analysis of G proteins using mutant endothelinA receptor.
66.	2002	Molecular mechanism for the activation of voltage-independent Ca²⁺ channels by endothelin-1 in Chinese hamster ovary cells stably expressing human endothelinA receptors.
67.	2002	Structural requirements for selective binding of ISC1 to anionic phospholipids.
68.	2001	Ca²⁺ channels activated by endothelin-1 in CHO cells expressing endothelin-A or endothelin-B receptors.
69.	2001	Characterization of Ca²⁺ channels involved in endothelin-1-induced mitogenic responses in vascular smooth muscle cells.
70.	2001	Failure of endothelin-1 to activate store-operated Ca²⁺ channels by lack of mobilization from intracellular Ca²⁺ stores in cultured bovine adrenal chromaffin cells.
71.	2000	Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells.
72.	2000	Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae.
73.	1999	Activation of three types of voltage-independent Ca²⁺ channel in A7r5 cells by endothelin-1 as revealed by a novel Ca2+ channel blocker LOE 908.
74.	1999	Pharmacological characterization of Ca²⁺ entry channels in endothelin-1-induced contraction of rat aorta using LOE 908 and SK&F 96365.
75.	1999	Pharmacological characterization of receptor-mediated Ca²⁺ entry in endothelin-1-induced catecholamine release from cultured bovine adrenal chromaffin cells.
76.	1998	Purification of a novel endothelin-converting enzyme specific for big endothelin-3.
77.	1998	Role of nonselective cation channels as Ca²⁺ entry pathway in endothelin-1-induced contraction and their suppression by nitric oxide
78.	1997	Palmitoylation of human endothelinB, Its critical role in G protein coupling and a differential requirement for the cytoplasmic tail by G protein subtypes.
79.	1997	Physiological role of Ca²⁺-permeable nonselective cation channel in endothelin-1-induced contraction of rabbit aorta.
80.	1997	The involvement of a novel mechanism distinct from the thrombin receptor in the vasocontraction induced by trypsin.
81.	1996	Endothelin-1-induced mitogenic responses of Chinese hamster ovary cells expressing human endothelinA: the role of a wortmannin-sensitive signaling pathway
82.	1995	Capacitative Ca²⁺ entry in human platelets is resistant to nitric oxide.
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■ Presentation

1.	2025/07/25	The Educational Effects of Kawasaki Medical School’s Research Laboratory Placement Program （Poster notice，General）
2.	2025/05/10	Sweat suppression promotes a Th2-biased sensitization to food allergens and anaphylaxis in an IL-6-dependent manner （Speech，General）
3.	2024/11/06	Analysis for the specificity of fluorescent substrates for acid ceramidase （Poster notice，General）
4.	2024/11/06	Functional analyses of glycerophosphodiesterase GDE4 in a prostate cancer cell line （Poster notice，General）
5.	2024/11/06	GPR176/Gz signaling is involved in fibroblast activation via the TGFβ1/Smads/αSMA pathway （Poster notice，General）
6.	2024/11/06	Investigation of the role of senescent cells in pulmonary fibrosis using p16-conditional knockout mice （Poster notice，General）
7.	2023/05/12	Sweat protects against allergic sensitization to food allergens （Poster notice，General）
8.	2023/03/14	Mechanism of decreased expression of the lipid mediator-degrading enzyme in fibrotic lung （Poster notice，General）
9.	2022/11/11	Glycerophosphodiesterase 7 produces cyclic phosphatidic acid in the ER lumen （Poster notice，General）
10.	2022/11/11	Role of senescent alveolar epithelial cells in pulmonary fibrosis （Poster notice，General）
11.	2022/06/26	Acid ceramidase as a third N-acylethanolamine hydrolase in cells and tissues （Poster notice，General）
12.	2022/03/28	Hydrolysis of anti-inflammatory and anorexic N-acylethanolamines by acid ceramidase as revealed by using saposin D-deficient mice （Poster notice，General）
13.	2022/03/08	Development of a simple assay to measure glycerophosphodiesterase GDE4 and GDE7 activities for screening their Inhibitors （Poster notice，General）
14.	2022/03/08	Hydrolytic activity of acid ceramidase on N-acylethanolamines, anti-inflammatory and anorexic lipid mediators: an analysis using saposin D-knockout mice （Poster notice，General）
15.	2022/03/08	The crosstalk between Il-4/IL-13 and S1P/S1P₂ signaling in phorbol ester-treated THP-1 MΦs （Poster notice，General）
16.	2022/03/07	Analysis of the role of macrophage senescence in lung fibrosis using macrophage-specific p16 knockout mice （Poster notice，General）
17.	2021/12/04	The sweating disturbance aggravates contact hypersensitivity reaction in mice footpads. （Speech，General）
18.	2021/11/03	Analysis of N-acylethanolamine-hydrolyzing activity using saposin D-deficient mice （Poster notice，General）
19.	2021/11/03	Fluorescence-based enzyme activity assay for glycerophosphodiesterase GDE4 and GDE7 （Poster notice，General）
20.	2021/11/03	S1P₂-Rho kinase pathway is necessary for full activation of STAT6 by IL-4/IL-13 in THP-1 macrophages （Poster notice，General）
21.	2021/11/03	The effect of p16 overexpression on gene expression of fibrosis-related factors in alveolar epithelial cells and macrophages （Poster notice，General）
22.	2021/04/27	Growth differentiation factor 15 activates macrophages and fibroblasts （Poster notice，General）
23.	2021/04/27	Role of acid ceramidase in the hydrolysis of anti-inflammatory and anorexic N-acylethanolamines （Poster notice，General）
24.	2021/04/27	The role of sphingosine 1-phosphate type 2 receptor on IL-4/IL-13-stimulated STAT6 phosphorylation in phorbol12-myristate13-acetate-treated THP-1 cells （Poster notice，General）
25.	2021/03/29	Changes in expression of fibrosis-related factors in p16 forced-expression cells （Poster notice，General）
26.	2021/03/09	Transcription factor ATF4 maintains pancreatic β-cell identity during ER stress （Speech，General）
27.	2021/03/08	Changes in expression of fibrosis-related factors in alveolar epithelial cells and macrophages by forced expression of p16 （Poster notice，General）
28.	2021/03/08	The role of sphingosine 1-phosphate receptor 2 on interleukin (IL)-4/IL-13-induced STAT6 phosphorylation in phorbol 12-myristate 13-acetate-stimulated THP-1 cells （Poster notice，General）
29.	2020/10/03	Inhibition of sphingolipid metabolism has ceramide-induced anti-tumor effect on pancreatic cancer cells. （Speech，General）
30.	2020/09/16	Acid ceramidase hydrolyzes N-acylethanolamines, a group of anti-inflammatory and anorexic lipid mediators, in cells and tissues （Speech，General）
31.	2020/03/17	Altered gene expression of lysophosphatidic acid receptor subtypes in ischemia/reperfusion-induced renal interstitial fibrosis in mice. （General）
32.	2020/03/17	Role of acid ceramidase in the degradation of N-acylethanolamines, a group of anti-inflammatory and anorexic lipid mediators. （Abstract Only，General）
33.	2019/06/19	Expression of lysophosphatidic acid receptor subtypes in ischemia/reperfusion-induced renal fibrosis in mice. （Poster notice，General）
34.	2019/06/19	Multiple pathways for N-acylethanolamine biosynthesis from N-acylphosphatidylethanolamine: tissue-dependent contribution of each pathway. （Poster notice，General）
35.	2019/03/23	The effect of growth/differentiation factor 15 (GDF15) on lung fibrosis. （Poster notice，General）
36.	2019/03/16	The role of growth/differentiation factor 15 (GDF15) in lung fibrosis. （Poster notice，General）
37.	2018/07/05	Microarray analysis in bleomycin-induced lung fibrosis. （Poster notice，General）
38.	2018/07/04	Biosynthesis of N-acylethanolamine and lysophosphatidic acid by glycerophosphodiesterase GDE4 and GDE7. （Poster notice，General）
39.	2018/07/02	Gender difference of vascular functional changes in diabetic condition. （Poster notice，General）
40.	2016/10/05	S1P2, a receptor for the lysophospholipid mediator sphingosine 1-phosphate, protects against vascular barrier disruption. （Speech，Panelist at Symposium/Workshop (Applied)）
41.	2015/07/13	Role of S1P2 in inflammation and fibrosis （Speech，Special/Invited Lecture）
42.	2015/06/17	Sphingosine 1-phosphate receptor-2 plays a protective role against anaphylaxis and acute lung injury （Poster notice，General）
43.	2014/04/16	Distinct role of S1P2 in the functional regulation of vascular endothelium and smooth muscle. （Speech，Panelist at Symposium/Workshop (Appointed)）
44.	2014/04/16	Sphingosine-1-phosphate receptor-2 (S1P2) negatively regulates eNOS and protects against acute vascular barrier disruption. （Poster notice，General）
45.	2013/01/29	ClassII PI3-kinase, PI3K-C2alpha, mediates S1P-induced endothelial cell migration through endosomal Rac1 activation. （Poster notice，General）
46.	2013/01/29	Endothelial PI3K-C2 alpha, a class II PI3K, has an essential role in angiogenesis and vascular barrier function. （Poster notice，General）
47.	2012/10/24	Class II PI3K-C2 alpha is essential for vascular barrier integrity. （Speech，Panelist at Symposium/Workshop (Other)）
48.	2012/07/25	Biosynthetic pathways of N-acylethanolamines from N-acylated plasmalogen phospholipid. （Poster notice，General）
49.	2009/07/10	The tumor suppressor H-rev107 functions as phospholipase A1/2 with a low N-acyltransferase activity. （Poster notice，General）
50.	2009/05/25	Characterization of the tumor suppressor H-rev107 as a novel Ca²⁺-independent cytosolic phospholipase A_1/2. （Speech，General）
51.	2009/05/25	The tumor suppressor H-rev107 functions as a novel Ca²⁺-independent cytosolic phospholipase A_1/2. （Poster notice，General）
52.	2007/06/27	Discovery of a calcium-independent phosphatidylethanolamine N-acyltransferase (iNAT). （Speech，General）
53.	2007/06/27	Immunochemical studies reveal predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages. （Poster notice，General）
54.	2006/11/25	Expression analysis of N-acylethanolamine-generating phospholipase D in rat brain. （Poster notice，General）
55.	2006/11/25	Molecular cloning and characterization of a novel lysosomal enzyme hydrolyzing anandamide and other bioactive N-acylethanolamines. （Poster notice，General）
56.	2006/06/25	Purification and characterization of recombinant NAPE-PLD expressed in Escherichia coli. （Poster notice，General）
57.	2006/06/21	Molecular characterization of a novel lysosomal enzyme hydrolyzing anandamide and other bioactive N-acylethanolamines. （Poster notice，General）
58.	2006/06/21	Purification and characterization of the recombinant anandamide-generating phospholipase D. （Poster notice，General）
59.	2006/05/26	Molecular biological studies on the anandamide-generating phospholipase D (NAPE-PLD). （Speech，Panelist at Symposium/Workshop (Appointed)）
60.	2005/06/24	Expression analysis of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D in rat brain regions. （Speech，General）
61.	2005/06/24	Molecular cloning and characterization of N-acylethanolamine-hydrolyzing acid amidase. （Speech，General）
62.	2004/10/06	Molecular cloning and expression of a novel phospholipase D releasing anandamide. （Speech）
63.	2004/06/23	Biosynthesis of anandamide and N-palmitoylethanolamine by sequential actions of phospholipase A₂ and lysophospholipase D. （Poster notice，General）
64.	2004/06/23	Molecular cloning and characterization of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD). （Speech，General）
65.	2003/06/25	Enzymatic studies on an anandamide biosynthetic pathway via N-acyl-lysophosphatidylethanolamine. （Speech，General）
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