ニシマツ　シンイチロウ   Shinichiro Nishimatsu   西松　伸一郎    所属   川崎医科大学  医学部 一般教養　自然科学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	A Wnt3a variant participates in chick apical ectodermal ridge formation: distinct biological activities of Wnt3a splice variants in chick limb development.
掲載誌名	正式名：Development, growth & differentiation 略　 称：Dev Growth Differ ISSNコード：00121592/1440169X
巻・号・頁	49(6),pp.493-501
著者・共著者	Narita Tomohiro, Nishimatsu Shin-ichiro, Wada Naoyuki, Nohno Tsutomu
担当区分	2nd著者
発行年月	2007/08
概要	Wnt/beta-catenin signaling is involved in the formation of the apical ectodermal ridge (AER) during vertebrate limb development. Although Wnt3a is a potent ligand for chick AER formation, whether chick Wnt3a can induce Fgf8 expression in chick embryos is unclear and the Wnt ligand involved in chick AER formation remains unknown. Here, we examined whether another Wnt3a isoform is expressed in the AER, and whether Wnt3 contributes to AER formation in chick as well as mouse embryos. We found that chick Wnt3 was not expressed in the presumptive limb ectoderm at the early stages of AER formation. Using 5'-rapid amplification of cDNA ends, we isolated another chick Wnt3a transcript. This novel variant, Wnt3a variant 2, induced Fgf8 in the limb ectoderm and activated the beta-catenin pathway in vivo and in vitro. These data showed that Wnt3a variant 2 is an active form of chick Wnt3a that regulates chick AER formation.
DOI	10.1111/j.1440-169X.2007.00938.x
文献番号	17488271