Shinichiro Nishimatsu    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Natural Sciences,    Position   Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Biochemical properties of amphibian bone morphogenetic protein-4 expressed in CHO cells.
Journal	Formal name：The Biochemical journal Abbreviation：Biochem J ISSN code：02646021/14708728
Volume, Issue, Page	291(2),pp.413-417
Author and coauthor	Suzuki Atsushi, Nishimatsu Shin-ichiro, Shoda Akihito, Takebayashi Kimiko, Murakami Kazuo, Ueno Naoto
Authorship	2nd author
Publication date	1993/04
Summary	The biochemical properties of recombinant amphibian bone morphogenetic protein-4 (BMP-4), the cDNA of which has been cloned recently by screening of a Xenopus cDNA library, was characterized. The protein was expressed by the transfection of Chinese hamster ovary (CHO) cells with the cDNA cloned into expression vectors bearing a cytomegalovirus promoter or a simian virus 40 promoter. Northern-blot analysis showed that the latter vector was more efficient for Xenopus BMP-4 expression. Specific antiserum against Xenopus BMP-4 peptide demonstrated that the protein is synthesized as a large precursor, processed to the mature form and then secreted from the cells as a homodimer. Analysis of the biological activity in the conditioned medium revealed that Xenopus BMP-4 has a potent alkaline phosphatase-inducing activity on mouse osteoblastic cells.
Document No.	8387268