ニシマツ　シンイチロウ   Shinichiro Nishimatsu   西松　伸一郎    所属   川崎医科大学  医学部 一般教養　自然科学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Biochemical properties of amphibian bone morphogenetic protein-4 expressed in CHO cells.
掲載誌名	正式名：The Biochemical journal 略　 称：Biochem J ISSNコード：02646021/14708728
巻・号・頁	291(2),pp.413-417
著者・共著者	Suzuki Atsushi, Nishimatsu Shin-ichiro, Shoda Akihito, Takebayashi Kimiko, Murakami Kazuo, Ueno Naoto
担当区分	2nd著者
発行年月	1993/04
概要	The biochemical properties of recombinant amphibian bone morphogenetic protein-4 (BMP-4), the cDNA of which has been cloned recently by screening of a Xenopus cDNA library, was characterized. The protein was expressed by the transfection of Chinese hamster ovary (CHO) cells with the cDNA cloned into expression vectors bearing a cytomegalovirus promoter or a simian virus 40 promoter. Northern-blot analysis showed that the latter vector was more efficient for Xenopus BMP-4 expression. Specific antiserum against Xenopus BMP-4 peptide demonstrated that the protein is synthesized as a large precursor, processed to the mature form and then secreted from the cells as a homodimer. Analysis of the biological activity in the conditioned medium revealed that Xenopus BMP-4 has a potent alkaline phosphatase-inducing activity on mouse osteoblastic cells.
文献番号	8387268